E. James Petersson

Petersson

Associate Professor of Chemistry

215-746-2221

350 N

Website

Awards

• Searle Scholar (2010)

• Sloan Research Fellow (2012)

• NSF CAREER Award (2012)

• Award for Early Excellence in Physical Organic Chemistry (2013)

• Institute on Aging Fellow (2014)

• Thieme Chemistry Journal Award (2017)

• Jane Glick Award for Graduate Teaching (2018)

Education

• A.B. Dartmouth College (1998)

• Ph.D. California Institute of Technology (2005)

• NIH Postdoctoral Fellow, Yale University (2005-2008)

Research Interests

Protein folding and protein interactions underlie both proper function and disease in biological systems. Many receptor proteins signal through complex interactions and rearrangements, and some proteins, such as the Parkinson’s Disease protein α-synuclein, misfold into toxic conformations.Studying these protein motions not only aids our understanding of diverse biological phenomena, it also contributes to an important fundamental problem in biochemistry: understanding how proteins fold and change shape. The Petersson laboratory is developing tools to address questions of how peptides and proteins mediate cellular communication and how the cellular environment catalyzes protein misfolding, from detailed in vitrofolding studies to imaging in live animals. These tools include novel chromophores, which we synthesize and incorporate into proteins through unnatural amino acid mutagenesis and synthetic protein ligation. We apply these tools to several key disease areas through collaborations in the Perelman School of Medicine: preventing the acquisition of antibiotic resistance by bacteria, Parkinson’s Disease, and fluorescence-guided cancer surgery. In many cases, the balance between health and disease is governed by post-translational modifications, for which we study the enzymes that install them, both to understand their biological roles and to utilize them in synthetic protein modification. Finally, an area of particular interest in the Petersson laboratory is the introduction of thioamide modifications to the peptide backbone, which can serve as protein folding probes, or stabilizers for improved therapeutic peptides or in vivoimaging reagents.

Selected Publications

Minimal Chromophores to Monitor Protein Motions

Efficient Synthesis and In Vivo Incorporation of Acridon-2-ylalanine, a Fluorescent Amino Acid for Lifetime and Förster Resonance Energy Transfer/Luminescence Resonance Energy Transfer Studies

Lee C. Speight, Anand K. Muthusamy, Jacob M. Goldberg, John B. Warner, Rebecca F. Wissner, Taylor S. Willi, Bradley F. Woodman, Ryan A. Mehl, and E. James Petersson

J. Am. Chem. Soc13518806-18814 (2013)

DOI10.1021/ja403247j

 

Multicolor Protein FRET with Tryptophan, Selective Coumarin-Cysteine Labeling, and Genetic Acridonylalanine Encoding

John J. Ferrie,* Naoya Ieda,* Conor M. Haney, Christopher R. Walters, Itthipol Sungwienwong, Jimin Yoon, and E. James Petersson

Chem. Commun5311072-11075 (2017)

DOI10.1039/c7cc05492k

Selected as a Hot Article for 2017!

 

Using a Large Ensemble FRET Library with Multiple Probe Pairs to Drive Monte Carlo Simulations of Disordered α-Synuclein

John J. Ferrie, Conor M. Haney, Jimin Yoon, Buyan Pan, Yi-Chih Lin, Zahra Fakhraai, Elizabeth Rhoades, Abhinav Nath, and E. James Petersson

Biophys. J11453-64 (2018)

DOIhttp://dx.doi.org/

Featured on the Biophysical Journal website!

 

Toward a Molecular Understanding of Parkinson's Disease

Site-Specific Fluorescence Polarization for Studying the Disaggregation of α-Synuclein Fibrils by Small Molecules

Conor M. Haney,* Christina L. Cleveland,* Rebecca F. Wissner,* Lily Owei, Jaclyn Robustell, Malcolm J. Daniels, Merve Canyurt, Priscilla Rodriguez, Harry Ischiropoulos, Tobias Baumgart, and E. James Petersson

Biochemistry 56683-691(2017)

DOI10.1021/acs.biochem.6b01060

Selected for the Biochemistry journal cover and highlighted in Biochemistry Newsflash!

 

Selective imaging of internalized proteopathic α-synuclein seeds in primary neurons reveals mechanistic insight into transmission of synucleinopathies

Richard J. Karpowicz Jr., Conor M. Haney, Tiberiu S. Mihaila, Raizel M. Sandler, E. James Petersson and Virginia M.-Y. Lee

J. Biol. Chem29213482-13497 (2017)

DOI:10.1074/jbc.M117.780296

Selected an Editor's Pick in J. Biol. Chem and highlighted by Health Medicine Network, Science Newsline, Medical Xpress, Newswise, EurekAlert!, and ALZForum.

 

Thioamide Modifications of the Protein Backbone

Thioamide Quenching of Fluorescent Probes through Photoinduced Electron Transfer: Mechanistic Studies and Applications

Jacob M. Goldberg, Solongo Batjargal, Benson S. Chen, and E. James Petersson

J. Am. Chem. Soc. 13518651-18658 (2013)

DOI10.1021/ja409709x

 

The Effects of Thioamide Backbone Substitution on Protein Stability: A study in α-helical, β-sheet, and polyproline II helical contexts

Christopher R. Walters, D. Miklos Szantai-Kis, Yitao Zhang, Zachary E. Reinert, W. Seth Horne David M. Chenoweth, and E. James Petersson

Chem. Sci82868-2877 (2017)

DOI10.1039/C6SC05580J

 

Thioamide Substitution Selectively Modulates Proteolysis and Receptor Activity of Therapeutic Peptide Hormones

Xing Chen, Elizabeth G. Mietlicki-Baase, Taylor M. Barrett, Lauren E. McGrath, Kieran Koch-Laskowski, John J. Ferrie, Matthew R. Hayes, and E. James Petersson

J. Am. Chem. Soc. 13916688–16695 (2017)

DOI10.1021/jacs.7b08417

Highlighted in Nature!

 

Post-Translational Modification Enzymology and Applications

Structure and Mechanism of Acetylation by the N-Terminal Dual Enzyme NatA/Naa50 Complex

Sunbin Deng, Robert S. Magin, Xuepeng Wei, Buyan Pan, E. James Petersson, and Ronen Marmorstein

Structure 271057-1070 (2019)

DOI10.1016/j.str.2019.04.014

 

Transferase-Mediated Labeling of Protein N-Termini with Click Chemistry Handles

Anne M. Wagner, John B. Warner, Haviva E. Garrett, Christopher R. Walters, and E. James Petersson

Methods Mol. Biol. 1337109-127 (2015)

DOI10.1007/978-1-4939-2935-1_15

 

*Authors contributed equally.

Affiliations