Biological Chemistry seminar: Yi-Wei Chang, University of Pennsylvania

February 14, 2019 - 03:00 PM - 04:00 PM

Yi-Wei Chang

University of Pennsylvania


"Structural biology in situ by cellular electron cryotomography"

Electron cryotomography (ECT) enables intact cells to be visualized in 3D in an essentially native state to macromolecular (~4 nm) resolution. It has allowed us to visualize the structures of macromolecular machines in their native context inside intact cells. In many cases, such machines cannot be purified intact for in vitro study. In other cases, the function of a structure is lost outside the cell, so that the mechanism can be understood only by observation in situ. In this presentation, I will use bacterial secretion systems as examples to show how ECT can help us to understand important biological processes by doing structural biology directly inside cells.


Carolyn Hoff Lynch Room

Cret, Chemistry Complex

Department of Chemistry

231 S. 34 Street, Philadelphia, PA 19104-6323

215.898.8317 voice | 215.573.2112 fax |