Biological

Sergei Vinogradov

Photo: 
First Name: 
Sergei
Last Name: 
Vinogradov
Official Title: 
Associate Professor of Biochemistry and Biophysics

Biophysical Chemistry, Photochemistry/Photophysics

Contact Information
Email: 
VINOGRAD@MAIL.MED.UPENN.EDU
Phone: 
215-573-7524
Education: 

M.S. (Chemistry) Moscow State University, Russia, 1988.

Ph.D. (Organic Chemistry) Moscow State University, Russia, 1995.

Research Interests: 
Dr. Vinogradov's research is focused on the development of advanced probes for microscopy and imaging applications. On the fundamental level our interests encompass chemistry of porphyrins and other pyrrolic dyes, energy and electron transfer in multichromophoric systems, spectroscopy and imaging. Essentially, we are a group of synthetic and physical chemists developing new techniques for biomedical research. Over the past years the main focus of the lab has been optical imaging of oxygen in biological systems, including chemistry of imaging probes, phosphorescence lifetime imaging instrumentation, image reconstruction methods and a variety of applications of phosphorescence. Other bio-analytes of interests have been pH and metal ions. Currently the laboratory also pursues interests in optical energy upconversion and magnetic field effects on luminescence in view of their applications in imaging. Dr. Vinogradov collaborates broadly with laboratories across the world whose interests include basic studies of cellular metabolism and applications in neuroscience, stem cell biology, cancer therapy, tissue engineering and ophthalmology.
Selected Publications: 

Esipova, T. V., Ye, X. C., Collins, J. E., Sakadzic, S., Mandeville, E. T., Murray, C. B., Vinogradov, S. A.: Dendritic upconverting nanoparticles enable in vivo multiphoton microscopy with low-power continuous wave sources. PNAS 109(51): 20826-20831, 2012.

 

Mani, T., Tanabe, M., Yamauchi, S., Tkachenko, N. V., Vinogradov, S. A.: Modulation of Visible Room Temperature Phosphorescence by Weak Magnetic Fields. Journal of Physical Chemistry Letters 3(21): 3115-3119, 2012.

 

Mani, T., Niedzwiedzki, D.M., Vinogradov, S.A.: Generation of Phosphorescent Triplet States via Photoinduced Electron Transfer: Energy and Electron Transfer Dynamics in Pt Porphyrin-Rhodamine B Dyads. J. Phys. Chem. A. 116: 3598−3610, 2012.

 

Vinogradov, S.A., Wilson, D.F. : Porphyrin-dendrimers as biological oxygen sensors. Designing dendrimers. Wiley, 2012.

 

Esipova, T. V., Karagodov, A., Miller, J., Wilson, D. F., Busch, T. M., Vinogradov, S. A.: Two new "protected" oxyphors for biological oximetry: properties and application in tumor imaging. Analytical Chemistry 83(22): 8756-8765, 2011.

 

Lecoq, J., Parpaleix, A., Roussakis, E., Ducros, M., Houssen, Y. G., Vinogradov, S. A.*, Charpak, S.*: Simultaneous two-photon imaging of oxygen and blood flow in deep cerebral vessels. Nature Medicine 17(7): 893-U262, 2011 Notes: Note that postdoc E. Roussakis is a co-first author and I am one of the two senior corresponding authors. My lab is entirely responsible for the new oxygen microscopy technology and worked with the collaborator to implement it in the brain.

 

Finikova, O. S., Lebedev, A. Y., Aprelev, A., Troxler, T., Gao, F., Garnacho, C., Muro, S., Hochstrasser, R. M., Vinogradov, S. A.: Oxygen microscopy by two-photon-excited phosphorescence. ChemPhysChem 9(12): 1673-1679, 2008.

 

Apreleva, S. V., Wilson, D. F., Vinogradov, S. A.: Tomographic imaging of oxygen by phosphorescence lifetime. Applied Optics 45(33): 8547-8559, 2006.

Hirschmann Visiting Professor - Lecture III (Sam Gellman, Wisconsin)

Thu, 2016-04-07 12:00 - 13:00
Speaker: 

Prof. Sam Gellman

University of Wisconsin, Madison

 

Location: 

Lynch Lecture Hall

 

Lecture II

Hirschmann Visiting Professor - Lecture II (Sam Gellman, Wisconsin)

Wed, 2016-04-06 13:00 - 14:00
Speaker: 

Prof. Sam Gellman

University of Wisconsin, Madison

Location: 

Lynch Lecture Hall

Lecture II

Biological Chemistry Seminar, Eva-Maria S. Collins , UCSD

Thu, 2016-02-25 15:00 - 16:00
Location: 
Carolyn Hoff Lynch Room

Special Biological Chemistry Seminar (Stavroula Hatzios, Harvard Medical School)

Fri, 2016-02-19 13:00 - 14:00
Location: 

Lynch Lecture Hall Chemistry Complex

Inquires please contact Camille Pride at campride@sas.upenn.edu

 

Special Biophysical Chemistry Seminar: Christy Landes, Rice

Wed, 2016-01-20 13:00 - 14:00
Speaker: 

Prof. Christy Landes

Rice University

Location: 
Lynch Lecture Hall
Attached Document: 

Single molecule dynamics at soft interfaces: from basic science to a $100,000,000,000 problem

 

Abstract

Special Biological Chemistry Seminar, Franziska Bleichert, John Hopkins University School of Medicine

Tue, 2016-01-12 13:00 - 14:00
Location: 

Carolyn Hoff Lynch Room

Title: Molecular mechanisms for initiating DNA replication in eukaryotes

Abstract:

Cellular organisms must replicate their DNA in a timely and accurate manner to preserve gene copy number, chromosome ploidy, and genome stability. In eukaryotes, DNA replication is initiated at specific sites throughout the genome, termed replication origins, which are bound by a multi-subunit protein assembly, the origin recognition complex (ORC). Aided by accessory factors, ORC facilitates loading of the replicative helicase core onto DNA to “license” origins for subsequent DNA replication.

In my seminar, I will describe how we used single-particle electron microscopy and X-ray crystallography to gain molecular insights into how the ORC subunits co-assemble, how ORC engages origin DNA, and how ORC interacts with partner proteins. I will discuss structure-guided models for how ORC operates during replicative helicase loading, as well as a novel mechanism for controlling metazoan ORC activity that may help regulate the onset of DNA replication in vivo. In addition, I will highlight how mutations in ORC subunits link ORC assembly defects to primordial dwarfism in humans.

 

Department of Chemistry

231 S. 34 Street, Philadelphia, PA 19104-6323

215.898.8317 voice | 215.573.2112 fax | web@chem.upenn.edu

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